Psilocybin QTest® The First Psilocybin Test Kit for Psilocybin Potency
Have you ever wondered, "how strong are my magic mushrooms?" Psilocybin potency varies wildly — between strains, and even from mushroom to mushroom in the same batch.
That's why we created the Psilocybin QTest® — the world's first scientifically validated psilocybin test kit, designed to measure the exact psilocybin content of your mushrooms in about 30 minutes, from the comfort of your home.
Invented and written by Dr. Felix Blei, the scientist who first elucidated psilocybin biosynthesis. Reviewed by Dr. Marina Garcia Moreno, Chief Scientific Officer at miraculix
Quick Overview
Quick Overview: Psilocybin QTest®
What is the Psilocybin QTest? The Psilocybin QTest® is the world's first scientifically validated, quantitative test kit that measures the exact psilocybin content of dried mushrooms, truffles and mycelium — not just whether psilocybin is present, but how much.
How accurate is it? Validated against HPLC to within ±10%, following the ICH Q2(R1) pharmaceutical validation guideline — the same standard used to verify active ingredients in medicine.
How does it work? A 0.15 g dried sample is extracted, incubated with the detection solution, and the resulting color is compared to a reference chart (or read precisely with the QTest Photometer) — giving a result in mg/g or % potency in about 30 minutes.
Does potency really vary that much? Yes. Even mushrooms of the same species and batch can differ several-fold in psilocybin content — see our own lab data below.
Where can I buy it? Available as a single-use kit or in multi-packs via our EU and US shops, with an optional photometer for lab-grade precision.
Who's behind it? The Psilocybin QTest® was invented by Dr. Felix Blei — still an active researcher in the field, most recently as co-author of a 2026 ChemBioChem study on Psilocybe cubensis chemistry.
Why use an Psilocybin Test Kit?
In a world where psychedelics are increasingly recognized for their therapeutic benefits and much more, having access to reliable, scientific data on mushroom potency is more important than ever. At miraculix, we’re proud to offer a solution that empowers users to take control of their psychedelic experiences in a safer, informed way. The Psilocybin QTest kit provides the accuracy and confidence needed for a safer psychedelic journey.
Psilocybin Content Varies — That's Why You Need to Test
Psilocybin content in magic mushrooms varies dramatically — by species, strain, growing conditions, and even between individual mushrooms in the same batch. Published research values can differ significantly from what we consistently measure with the Psilocybin QTest®.
The table below compares literature values with our own lab-tested results — in several cases, our measurements run notably higher.
Psilocybin Content by Species
| Species | Literature | Our Lab Data (University FSU Jena and Drugchecking) |
|---|---|---|
| P. cubensis | 0.63 % ¹ | 0.5–2.8 % |
| P. semilanceata | 0.98 % ¹ | 1.5 % |
| P. cyanescens | 0.85 % ² | 1–2.4 % |
| P. mexicana (fruiting body) | 0.2–0.4 % ³ | 0.9–1.7 % |
| P. mexicana (sclerotia) | no reliable data | 0.3–0.6 % |
¹ Gartz, 1994 · ² Stijve & Kuyper, 1985 · ³ Hofmann et al., 1959
Cultivation, extraction methods, and detection technology have all improved since these studies — but that only proves the point: no printed number tells you what's in your sample. Only a potency test does.
What the Psilocybin Test Kit Can Do
- Rapidly know how strong your mushrooms are
- Minimal sample required, user-friendly testing
- Grind up a whole batch and just test one sample
- University validated method
- The only mushroom potency test on the market
Easy 3-Step Process
The Psilocybin Test Kit provides a quick and simple way to measure psilocybin potency in just three steps. Whether at home or in a lab, the Psilocybin QTest delivers accurate results in minutes.
- Step 1: Preparation
Place a small sample (0.15 grams) of dried mushrooms into the extraction vial. - Step 2: Extraction
Add extraction solution to isolate the psilocybin for precise measurement. - Step 3: Reaction & Results
Compare the color change to the reference chart, which ranges from 0% to 2.4% potency.
This simple process ensures accurate, reliable results every time with the Psilocybin Test Kit.
Easy-to-Read Results
The Psilocybin Test Kit offers quick visual feedback with color changes in the vials, allowing you to easily identify the psilocybin potency of your sample. Whether it's no psilocybin, a light concentration, or a high potency, the included reference chart ensures accurate measurement, from 0% to 2.4% psilocybin.
Immediate Feedback: Instantly determine if your sample has no psilocybin, low potency, or strong psilocybin concentration.
Clear Color Indicators: The vials show distinct color changes, ranging from clear to deep red, indicating varying psilocybin levels.
Precise Measurement: Use the reference chart for an accurate and reliable reading of psilocybin potency.
How the Psilocybin Test Kit Measures Mushroom Potency
The Psilocybin Test Kit works via a colorimetric scale, where each color corresponds to a specific psilocybin potency level. This makes it easy to assess the potency of your mushroom sample without the need for complex equipment. Simply compare the sample’s color reaction to the reference chart, and you’ll get a clear visual estimate of the psilocybin concentration, from 0 % to 2.4 %.
| Psilocybin Potency (%) | Color Description |
| 0 % (0 mg) | clear, slight yellow tone |
| 0,6 % (6 mg) | light brown |
| 1,2 % (12 mg) | brown |
| 1,8 % (18 mg) | dark brown |
| 2,4 % (24 mg) | almost black |
What is inside the Psilocybin Test Kit
- Box with Psilocybin QTest label
- Evaluation scale with quick-reference guide
- Detailed instructions in various languages
- Glass vessel with detection solution
- Plastic vessel with extraction solution
- 1 ml syringe
- Blunt needle
- Sterifilt (filter for extraction)
There is also a PRO VERSION
Whether you're an experienced user, a grower, or a scientist, our affordable QTest-Photometer is designed to meet your needs. It is a precalibrated photometer which display you a precise amount of psilocybin, and works with other QTests as well.
Wanna test larger quantities? We offer solutions for that too! In combination with our spectrophotometer, we’ve developed the Psilocybin BulkQTest, designed specifically for use with the QTest photometer. This kit is perfect for high-precision analysis while keeping costs low.
US VERSUS THEM
What distinguishes our Psilocybin potency test from qualitative psilocybin test kits such as Ehrlich reagent when testing magic mushrooms:
- Quantiative Results in 30min
- Precise detection of Psilocybin and Psilocin
- Reliable across all natural sample types
- Accurate at various concentrations
Test Psilocybin Mushroom Potency in 3 easy steps
Preparation and Extraction
To begin testing your psilocybin sample, start by finely chopping and homogenizing approximately 0.15 grams of your dried mushroom material. Once the material is evenly ground, add it to the solvent reagent included in your psilocybin test kit for extraction. This step ensures that the psilocybin compounds are properly dissolved and ready for accurate testing, allowing you to measure the potency of your mushrooms with precision.
Incubation and Detection
After preparing your sample, add 1 ml of the extraction solution to the detection vial provided in your psilocybin potency test kit. Next, incubate the vial in hot water to ensure optimal conditions for the detection reaction. This crucial step helps to activate the reagents, allowing for an accurate measurement of psilocybin potency in your sample.
Evaluation of Potency
Compare the Color Intensity: Once the reaction is complete, compare the color intensity in your detection vial with the provided color chart. This will help you accurately determine the psilocybin potency of your mushrooms using the psilocybin test kit. The more intense the color change, the higher the concentration of psilocybin in your sample, allowing for a precise and reliable assessment of its potency.
Step-by-step Video for Determining Magic Mushrooms
In this video, we show you all the components that you will receive when you are supplied with a Psilocybin QTest - the first psilocybin potency test. We also show you all the steps involved in carrying out the quick test. We have used different mushrooms containing psilocybin to show the differences and fluctuations in the active ingredient content. This can be seen particularly well in the evaluation step.
The Science Behind: Validated and Proven Accuracy
- Scientifically Validated: The Psilocybin QTest is the first scientifically validated psilocybin potency test kit available for home use.
- Lab-Grade Accuracy: Our test has been rigorously compared to professional lab methods like HPLC (High-Performance Liquid Chromatography), providing you with results you can trust.
- Trusted by Experts: Leading mycologists and researchers, like Alan Rockefeller, rely on the PSILOCYBIN QTest for testing rare mushroom species and conducting in-depth studies.
Our Psilocybin potency test has undergone thorough validation to ensure accuracy and reliability. The test follows the ICH Q2(R1) pharmaceutical validation guidelines, which are typically used to ensure the safety and precision of active ingredients in human medicine. This means you can trust the potency measurements provided by our test kit. To see the full validation data and comparison curve, click here to view the validation sheets.
CASE Report
Psilocybin Test Kit Validation and Case Report
In this Case Report, we demonstrate the precision and reliability of the Psilocybin Test Kit for measuring psilocybin potency. Dried Psilocybe cubensis Albino AA+ fruiting bodies were first finely ground. Different amounts of this powdered biomass were accurately weighed and placed into separate Eppendorf tubes for extraction.
Each sample was individually extracted and filtered, then incubated with the test kit's detection solution in cuvettes. After incubation, the samples were measured using the QTest Photometer at 595 nm, providing accurate results based on the color change.
Simultaneously, three 50 mg samples were extracted and analyzed using HPLC to determine the exact psilocybin content, ensuring the accuracy of the results.
The resulting plot shows a highly linear correlation between the color change and psilocybin concentration, confirming the effectiveness of the Psilocybin QTest. The data exhibits a strong linear relationship (R² = 0.9984), validating the Psilocybin Test Kit as a reliable method for measuring psilocybin content.
This experiment highlights the precision and reliability of the Psilocybin Potency Test, demonstrating how the Psilocybin QTest provides an easy-to-use, scientifically backed solution for determining mushroom potency with confidence.
Homogenization of dried fruiting bodies from Psilocybe cubensis "AA+"
Ascending amounts of the sample in each Eppendorf tube
Perfect color series after incubation with detection solution
Plot shows nearly perfect linearity of the Psilocybin Test kit
Empower Safer Choices
Are You Convinced?
Buy a Psilocybin Test Kit now
and directly contribute to new research and the development of new QTest kits.
With every purchase in our webshop, you’re supporting a small independent university start up and advancing harm reduction efforts and psychedelic research. Our mission is to empower everyone to make informed, self-determined decisions with trustworthy, lab-grade test kits.
A tool for Science & More
The Psilocybin test kit is more than just a psilocybin potency test—it's a tool that is being used for a community research project. In collaboration with Charité – Universitätsmedizin Berlin, this project allows users to anonymously submit their results and experiences, contributing to the progress of psychedelic science. Your data helps us explore how psilocybin content varies across different species and strains, with the aim of publishing the findings as open-source for the scientific community.
Knowledge is power! By joining this mission and collaborating together, we can finally answer many open questions and connect the missing pieces of evidence.
New and Trending Strain Names: Namaste, Chupacabara, Deep End & More
Beyond the classic strains above, grower communities and cultivation competitions continue to introduce new named cultivars — strains like Namaste, Creature, Tasmanian Cubensis, Inca Stargazer, Deep End, Chupacabara, and Red Boys regularly appear in grower forums and events such as the Psilocybin Cup.
As with most cultivated Psilocybe cubensis strains, potency claims for these newer names are largely self-reported by growers rather than independently verified through peer-reviewed analysis. The same principle from this article applies here too: a strain name alone — however striking — tells you very little about actual psilocybin content.
If you're growing or working with one of these newer cultivars, the only way to know its real potency is testing it yourself. See how the Psilocybin QTest works →
Behind Miraculix: Dr. Felix Blei and the Development of the Psilocybin Potency Test Kit
Dr. Felix Blei, CEO and founder of miraculix, began his career researching psilocybin and other psychoactive fungi, earning his PhD in microbiology. His groundbreaking work on the biosynthesis of psilocybin in Psilocybe mushrooms laid the foundation for the Psilocybin Test Kit named Psilocybin QTest. As the first to fully elucidate the psilocybin biosynthetic pathway, Dr. Blei's discoveries paved the way for the development of quantitative psilocybin testing.
In 2020, Dr. Blei founded Miraculix to apply his research to practical solutions. The Psilocybin QTest was created as the first reliable test for psilocybin potency, empowering users to safely measure the strength of their mushrooms. With his extensive background in natural product biosynthesis, Dr. Blei’s work continues to contribute to harm reduction and safer psychedelic use worldwide.
Through miraculix, Dr. Blei bridges cutting-edge research with real-world applications, providing tools for both researchers and users to better understand psilocybin and its effects.
Psilocybin Blogs
Explore More with the Psilocybin Test Kit Blog
The psilocybin test kit is more than just a tool — it’s your key to understanding mushroom potency, storage safety, and responsible use. In our latest blog highlights, you’ll learn how to spot spoiled mushrooms, how long psilocybin lasts, and whether it can be detected in a drug screening. Whether you’re using the mushroom test kit to analyze homegrown shrooms or check aged samples for potency loss, these expert guides provide the insights you need to stay informed and safe.
Do Magic Mushrooms Go Bad? Learn How to Spot Spoilage & Test Potency
Not sure if your old stash is still safe or strong? Discover how psilocybin degrades, what blue bruising really means, and how to detect spoiled mushrooms before it’s too late. This guide breaks down storage tips, mold warnings, and how the psilocybin test kit helps you check for potency loss at home.
Magic Mushroom Knowledge Hub: Cultivation, Chemistry & Psilocybin Testing
From growing your own mushrooms to understanding how psilocybin works—our Magic Mushroom Hub covers it all. Learn how to safely use the psilocybin test kit, understand brain effects, legal status, microdosing, and how to identify different strains.
Psilocybin Drug Tests: Can You Be Detected? Learn the Facts
Worried about drug tests after a mushroom trip? Learn what types of drug screenings can detect psilocybin or psilocin—and why most standard tests won’t. This article explains detection windows, metabolic breakdown, and how it differs from the psilocybin test kit.
Frequently asked questions about the miraculix
psilocybin mushroom test kit
Got questions about the QTest® for magic mushroom potency? We’ve got answers.
If you are looking for a reliable mushroom test kit, this FAQ covers everything you need to know about the Psilocybin QTest®.
From how it works to what it detects (and doesn’t), find all essential details below.
The Psilocybin QTest is the first scientifically validated home-use psilocybin test kit designed to measure the psilocybin potency of magic mushrooms. It provides accurate, reliable data on the psilocybin and psilocin content in your mushrooms in just 30 minutes.
Absolutely! The PSILOCYBIN QTest is perfect for testing the potency of any psilocybin-containing mushrooms, including popular species like Psilocybe cubensis, truffles, and even mycelium. It requires only 0.15 g of dried material to perform the test.
No, the PSILOCYBIN QTest is specifically designed for use with dried fungal materials, such as mycelium, fruiting bodies, or truffles. Fresh mushrooms contain a high and fluctuating water content, which can interfere with accurate measurements in this psilocybin test.
Yes. The Psilocybin QTest can also be used with most solvent-free mushroom extracts.
Because extracts are usually more concentrated than dried mushroom material, using the standard 150 mg sample amount may produce a color that is too intense for the evaluation chart. To keep the result within the readable color range, use less material depending on how concentrated your extract is.
Before testing, make sure that the extract is well homogenized and as solvent-free as possible. Residual alcohol or other solvents may interfere with the extraction solution and affect the result.
As a general guideline:
- For a 3× concentrated extract, use 50 mg (0.05 g) of material.
- For a 10× concentrated extract, use 15 mg (0.015 g) of material.
After comparing the color with the evaluation chart, multiply the displayed result by the concentration factor of your extract.
For example, if a 3× extract gives a result of 12 mg/g on the color chart, the concentration in the original sample is 36 mg/g.
No, the PSILOCYBIN QTest is specifically designed to measure psilocybin and psilocin content in magic mushrooms and does not work with synthetic substances like 4-AcO-DMT.
The PSILOCYBIN QTest quantifies the total alkaloid content (psilocybin + psilocin) and gives the added values in the form of a total concentration of psilocybin.
The background for this is that only the very stable active ingredient psilocybin is naturally present in healthy fungal tissue. For example, if not properly dried and stored, degradation of the prodrug psilocybin to unstable psilocin, the actual pharmacological active ingredient, occurs. Even if this occurs, the PSILOCYBIN QTest will give you information about the total amount of both alkaloids. Both substances have different molecular weights and to avoid a complicated conversion we give the value in terms of the well-known alkaloid psilocybin.
The PSILOCYBIN-QTEST works well with, for example, rotated or evaporated ("dry") extracts, but also some solvents like ethanol work if the concentration of alkaloids is not too low. This means that if too many foreign solvents are added to our extraction, it will disturb our extraction system.
The best way would be to evaporate the ethanol or any other solvent from a defined amount, note the dry weight, and measure it with the test.
The PSILOCYBIN QTest has a shelf life of at least 18 months if stored properly. Keep the reagents in the refrigerator at (35 - 46 °F/ 2 - 8 °C) and out of direct sunlight. Over time, the extraction liquid may turn slightly yellow, that’s okay. It is still usable.
Yes we have a validation for our PSILOCYBIN-QTests. This is a voluntarily validated and standardized test procedure based on the pharmaceutical method validation guideline ICH Q2(R1), which we have extended to the QTests. This guideline describes the requirements of the analytical method for active ingredients that are subsequently to be used in human medicine and guarantees a safe dosage of these active ingredients, for example in pharmacies.
You can find the short report Validation here: Validation report
Not quite. Many products called "mushroom test kits" are designed to identify toxic wild mushrooms or confirm species, not measure potency. The Psilocybin QTest® is different — it's a quantitative mushroom test kit built specifically to measure how much psilocybin is actually in your sample, giving you an exact concentration rather than just a species identification.
Yes. The Psilocybin QTest® works on any dried psilocybin-containing mushroom, truffle, or mycelium — not just Psilocybe cubensis. As long as the material is dried and properly homogenized, the test kit accurately measures total psilocybin content regardless of species.
Yes and yes! We would like to offer access to our instructions at any time. That is why we offer the option of viewing the instructions digitally and using them for execution. We would also like to expand worldwide access and make additional languages available. To this end, we are in the process of translating the individual instructions. You can find the current instructions for our test kit here:
English: Psilocybin QTest Manual EN
German: Psilocybin QTest Manual DE
Spanish: Psilocybin QTest Manual ES
Portuguese: Psilocybin QTest Manual PT
Dutch: Psilocybin QTest Manual NL
Polish: Psilocybin QTest Manual PL
French: Psilocybin QTest Manual FR
Czech: Psilocybin QTest Manual CZ
Italien: Psilocybin QTest Manual IT
We recommend ensuring that floating fungi are not sucked into the system and positioning the sterile filter below the floating fungus layer before applying vacuum. If the process fails initially, cleaning the filter very carefully and reapplying the syringe can help. The test can still function if fungal components are transferred, but this may complicate color evaluation. Link to the video.
No, the test is single-use. However, we offer multi-test packs if you need to test several batches or strains.
No, the Psilocybin QTest® is currently only available directly through our official EU and US webshops. Buying directly ensures you get an authentic, correctly stored kit and directly supports our ongoing research and harm reduction work — something a third-party marketplace listing can't guarantee.
You still have open questions about our Psilocybin test kit?
We could not answer your questions completely on this page? Then write us your questions about our psilocybin test kit quickly and easily in the form.
About the author
Dr. Felix Blei – Scientist and Scientific Author
Dr. Felix Blei is the CEO and Founder of miraculix Lab, and a Postdoctoral Researcher at the Friedrich Schiller University Jena. With a PhD (Dr. rer. nat.) in Microbiology and a strong background in natural product biosynthesis, he is internationally recognized for his pioneering work on psychoactive fungi—particularly the biosynthesis of psilocybin and related compounds (Blei, F., 2020).
During his doctoral research at the Institute of Pharmaceutical Microbiology under Prof. Dirk Hoffmeister, Dr. Blei was the first to elucidate the full biosynthetic pathway of psilocybin in Psilocybe mushrooms (Fricke, Blei et al., 2017) Angewandte Chemie. He further developed an in vitro system capable of producing psilocybin, serotonin, and novel non-natural analogues (Blei et al., 2018) Chemistry- A European Journal . His discovery of naturally occurring β-carbolines in Psilocybe—compounds that may synergistically enhance psilocybin's neurotropic effects—led to the concept of “psilohuasca,” a naturally occurring combination of MAO inhibitors and psychedelics in fungi (Blei, Dörner et al. 2020) Chemistry–A European Journal.
Building on his academic expertise, Dr. Blei developed the first reliable rapid tests for psilocybin, which laid the foundation for the spin-off company miraculix. Today, miraculix provides quantitative drug checking tools that are used throughout Germany and Europe. He also leads the German ALIVE project—an evidence-based drug checking Initiative funded by state governments, offering mobile substance analysis and harm reduction at music festivals and public events.
With over 500 citations, numerous publications in high-impact journals, and a passion for accessible science, Dr. Blei bridges cutting-edge research with real-world application. His work contributes to safer substance use, public health strategies, and a deeper understanding of natural psychoactive compounds.
You can learn more about him on LinkedIn or read his publications on Researchgate
Dr. rer. nat. Felix Blei
About Dr. Garcia Moreno
About the Reviewer: Dr. Marina Garcia Moreno
Dr. Marina Garcia Moreno is the Chief Scientific Officer at miraculix Lab, where she leads scientific development focused on harm reduction, drug checking, and psychoactive substance analysis. She holds a PhD (Dr. rer. nat.) in Medical Microbiology and Bacteriology from Friedrich Schiller University Jena (Germany), and has over 8 years of experience in biomedical and translational research.
Before joining miraculix, Dr. Garcia Moreno worked as a postdoctoral researcher at the University Clinic Jena. Her academic training includes a Master’s degree in Biomedicine and Molecular Biology from the University of the Basque Country.
As a multilingual science communicator, she is deeply committed to science-based education, public health, and bridging the gap between research and society. Through her writing and development of analytical tools, she aims to make scientific knowledge accessible, reliable, and actionable for a broad audience, from professionals to the general public.
You can learn more about her background on LinkedIn.
Dr. rer. nat. Marina Garcia Moreno
References
- Gartz, J. Extraction and analysis of indole derivatives from fungal biomass. J. Basic Microbiol. 1994, 34 (1), 17–22. DOI: 10.1002/jobm.3620340104
- Stijve, T.; Kuyper, T. W. Occurrence of psilocybin in various higher fungi from several European countries. Planta Med. 1985, 51 (5), 385–387. DOI: 10.1055/s-2007-969526
- Hofmann, A.; Heim, R.; Brack, A.; Kobel, H.; Frey, A.; Ott, H.; Petrzilka, T.; Troxler, F. Psilocybin und Psilocin, zwei psychotrope Wirkstoffe aus mexikanischen Rauschpilzen. Helv. Chim. Acta 1959, 42 (5), 1557–1572. DOI: 10.1002/hlca.19590420518
- Schober, S.; Dorfmann, L.; Walther, K.; Blei, F.; Chadeayne, A. R.; Gressler, M.; Bartram, S.; O'Connor, S. E.; Hoffmeister, D. Specific and Multi-Product Clade I and Clade IV Sesquiterpene Synthases Contribute to the Psilocybe cubensis Volatilome. ChemBioChem 2026, 27 (8), e70318. DOI: 10.1002/cbic.70318